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Nutrient Sensing in Eukaryotes

Redigeret af Fiona M. Russell, D. Grahame Hardie ...

Serier serie Springer Protocols
This detailed volume explores various methods through which nutrient sensing can be studied, particularly in multicellular eukaryotes. Beginning with a section on the monitoring of cellular energy status by sensing of adenine nucleotides, the book continues by delving into numerous techniques for researching both intracellular and extracellular nutrient sensing. Written for the highly successful ... Læs mere

1.428,99 kr.

This detailed volume explores various methods through which nutrient sensing can be studied, particularly in multicellular eukaryotes. Beginning with a section on the monitoring of cellular energy status by sensing of adenine nucleotides, the book continues by delving into numerous techniques for researching both intracellular and extracellular nutrient sensing. Written for the highly successful
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Mass Spectrometry-Based Proteomics

Redigeret af Kris Gevaert ...

Serier serie Springer Protocols
This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

1.414,11 kr.

This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by
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Antibody Engineering Volume 1

Redigeret af Roland E. Kontermann, Stefan Dübel ...

Serier serie Springer Protocols
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Læs mere

3.166,13 kr.

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new
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Applied Biocatalysis

The Chemist's Enzyme Toolbox

Redigeret af John Whittall, Peter W. Sutton ...

Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistryThis book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and ... Læs mere

1.609,86 kr.

Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistryThis book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and
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Heterologous Expression of Membrane Proteins

Methods and Protocols

Redigeret af Isabelle Mus-Veteau ...

Serier serie Springer Protocols
This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus ... Læs mere

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This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus
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Enzyme-Mediated Ligation Methods

Redigeret af Timo Nuijens, Marcel Schmidt ...

Serier serie Springer Protocols
This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and ... Læs mere

924,61 kr.

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and
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Mitochondrial Medicine

Volume 2: Assessing Mitochondria

Redigeret af Volkmar Weissig, Marvin Edeas ...

Serier serie Springer Protocols
This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Læs mere

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This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D
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Metabolic Reprogramming

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Redigeret af Salvatore Papa, Concetta Bubici ...

Serier serie Springer Protocols
This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide ... Læs mere

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This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide
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Therapeutic Proteins

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Redigeret af Millicent O. Sullivan, Bryce Chackerian, Wilfred Chen ...

Serier serie Springer Protocols
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This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the
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Redigeret af Salvatore Santamaria ...

Serier serie Springer Protocols
This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer. Written in the highly successful Methods in Molecular Biology series format, ... Læs mere

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Lysosomes

Methods and Protocols

Redigeret af Stephanie M. Hughes, Indranil Basak ...

Serier serie Springer Protocols
This volume provides a wide range of methods to discover and assess lysosome structure and function are provided. Chapters detail the essential methodologies and giving expert insight to key features with notes pertaining to key elements of the methods.Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of ... Læs mere

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This volume provides a wide range of methods to discover and assess lysosome structure and function are provided. Chapters detail the essential methodologies and giving expert insight to key features with notes pertaining to key elements of the methods.Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of
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Serier serie Springer Protocols
This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell ... Læs mere

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This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell
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Nutrient Sensing in Eukaryotes

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Mass Spectrometry-Based Proteomics

Antibody Engineering Volume 1

Applied Biocatalysis

Heterologous Expression of Membrane Proteins

Enzyme-Mediated Ligation Methods

Mitochondrial Medicine

Metabolic Reprogramming

Therapeutic Proteins

Proteases and Cancer

Lysosomes

Recombinant Protein Expression in Mammalian Cells