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Fluorescence In Situ Hybridization (FISH)

Application Guide

Redigeret af Thomas Liehr ...

Serier serie Springer Protocols
This third edition provides protocols on fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches. Chapters provide new and updated methods on probe, cell types, tissues, organisms, plants, microorganisms, multicolor FISH procedures, chromosomes, breakpoints, cryptic aberrations, nuclear architecture, epigenetic changes, and comparative genomic hybridization ... Læs mere

1.681,11 kr.

This third edition provides protocols on fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches. Chapters provide new and updated methods on probe, cell types, tissues, organisms, plants, microorganisms, multicolor FISH procedures, chromosomes, breakpoints, cryptic aberrations, nuclear architecture, epigenetic changes, and comparative genomic hybridization
... Læs mere
Fluorescence In Situ Hybridization (FISH) - Application Guide

Redigeret af Thomas Liehr ...

Serier serie Springer Protocols
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of ... Læs mere

1.428,99 kr.

This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of
... Læs mere

Genotoxicity Assessment

Methods and Protocols

Redigeret af Alok Dhawan, Mahima Bajpayee ...

Serier serie Springer Protocols
This third edition volume explores the latest advancements in genotoxicity testing and combines established assays with emerging technologies. Organized into five thematic sections, the book examines gene mutation assays, assays for chromosomal abnormalities, tests for primary DNA damage, assays in alternatives to animal models, as well as multipronged approaches to the assessment of genotoxicity. ... Læs mere

1.344,86 kr.

This third edition volume explores the latest advancements in genotoxicity testing and combines established assays with emerging technologies. Organized into five thematic sections, the book examines gene mutation assays, assays for chromosomal abnormalities, tests for primary DNA damage, assays in alternatives to animal models, as well as multipronged approaches to the assessment of genotoxicity.
... Læs mere
Poly(ADP-Ribose) Polymerase

Methods and Protocols

Redigeret af Alexei V. Tulin ...

Serier serie Springer Protocols
This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as ... Læs mere

1.400,85 kr.

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as
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Mitotic Exit

Methods and Protocols

Redigeret af Victor M. Bolanos-Garcia ...

Serier serie Springer Protocols
This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications ... Læs mere

1.840,86 kr.

This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications
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Heterologous Expression of Membrane Proteins

Methods and Protocols

Redigeret af Isabelle Mus-Veteau ...

Serier serie Springer Protocols
This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus ... Læs mere

1.400,85 kr.

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus
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Plant Immunity

Methods and Protocols

Redigeret af Tsutomu Kawasaki, Hirofumi Yoshioka ...

Serier serie Springer Protocols
This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, ... Læs mere

1.681,11 kr.

This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses,
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Single-Domain Antibodies

Methods and Protocols

Redigeret af Greg Hussack, Kevin A. Henry ...

Serier serie Springer Protocols
This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly ... Læs mere

1.681,11 kr.

This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly
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Chromosome Analysis

Methods and Protocols

Redigeret af Eisuke Gotoh ...

Serier serie Springer Protocols
This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome ... Læs mere

840,49 kr.

This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome
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Inclusion Bodies

Methods and Protocols

Redigeret af Julian Kopp, Oliver Spadiut ...

Serier serie Springer Protocols
This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent ... Læs mere

1.008,61 kr.

This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent
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DNA Damage Detection

Methods and Protocols

Redigeret af Takamitsu A. Kato ...

Serier serie Springer Protocols
This volume explores techniques in DNA damage analysis, important not only for understanding cellular health after exposure to genotoxic agents but also for assessing cellular ability of genome maintenance. The book explores traditional DNA damage measurement methods, as well as modern fluorescence-based analysis and high throughput automatic analysis methods. Written for the highly successful ... Læs mere

1.681,11 kr.

This volume explores techniques in DNA damage analysis, important not only for understanding cellular health after exposure to genotoxic agents but also for assessing cellular ability of genome maintenance. The book explores traditional DNA damage measurement methods, as well as modern fluorescence-based analysis and high throughput automatic analysis methods. Written for the highly successful
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Peroxisomes

Methods and Protocols

Redigeret af Michael Schrader ...

Serier serie Springer Protocols
This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast ... Læs mere

1.867,83 kr.

This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast
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Fluorescence In Situ Hybridization (FISH)

Fluorescence In Situ Hybridization (FISH) - Application Guide

Personer, der læser disse, syntes også om...

Genotoxicity Assessment

Poly(ADP-Ribose) Polymerase

Mitotic Exit

Heterologous Expression of Membrane Proteins

Plant Immunity

Single-Domain Antibodies

Chromosome Analysis

Inclusion Bodies

DNA Damage Detection

Peroxisomes