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Ewing Sarcoma

Methods and Protocols

Redigeret af Florencia Cidre-Aranaz, Thomas G.P. Grünewald ...

Serier serie Springer Protocols
This second edition volume aims at providing new and updated methods detailing the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters guide the readers through molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab ... Læs mere

1.868,14 kr.

This second edition volume aims at providing new and updated methods detailing the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters guide the readers through molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab
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IMMUNO-model in Cancer

Methods and Protocols

Redigeret af Sweta Rani, Lukasz Skalniak ...

Serier serie Springer Protocols
This open access volume describes the computational and experimental models that help researchers understand the responses of the immune system in cancer and test experimental immune-oncology approaches. The chapters in this book cover methods such as profile polarization in macrophages using ELISA; immune checkpoint inhibitors; interactions of PD-1/PD-L1 and T cells; immunocompetent preclinical ... Læs mere

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This open access volume describes the computational and experimental models that help researchers understand the responses of the immune system in cancer and test experimental immune-oncology approaches. The chapters in this book cover methods such as profile polarization in macrophages using ELISA; immune checkpoint inhibitors; interactions of PD-1/PD-L1 and T cells; immunocompetent preclinical
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Tertiary Lymphoid Structures

Methods and Protocols

Redigeret af Marie-Caroline Dieu-Nosjean ...

Serier serie Springer Protocols
This second edition details new and updated chapters on technologies related to tertiary lymphoid structures( TLS). Chapters guide readers through TLS in cancers, different stages of TLS development, immune function of TLS, molecular, cellular, and imaging methods, and preclinical methods to uncover the function of cells composing TLS in inflammatory situations in humans. Written in the highly ... Læs mere

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This second edition details new and updated chapters on technologies related to tertiary lymphoid structures( TLS). Chapters guide readers through TLS in cancers, different stages of TLS development, immune function of TLS, molecular, cellular, and imaging methods, and preclinical methods to uncover the function of cells composing TLS in inflammatory situations in humans. Written in the highly
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B-Cell Receptor Signaling

Methods and Protocols

Redigeret af Palash Chandra Maity ...

Serier serie Springer Protocols
B-lymphocytes produce antibodies, mediate humoral responses, and act as a key to the adaptive immune system of our body. The development and function of B cells are largely dependent on B cell antigen receptors (BCRs), which transmit a multitude of signals upon binding to the cognate antigen and activate the survival, proliferation, and differentiation of B cells thereof. Thus, studying the BCR ... Læs mere

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B-lymphocytes produce antibodies, mediate humoral responses, and act as a key to the adaptive immune system of our body. The development and function of B cells are largely dependent on B cell antigen receptors (BCRs), which transmit a multitude of signals upon binding to the cognate antigen and activate the survival, proliferation, and differentiation of B cells thereof. Thus, studying the BCR
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Circulating Tumor Cells

Methods and Protocols

Redigeret af Mark Jesus Magbanua ...

Serier serie Springer Protocols
This second editions explores new and updated approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Chapters detail comprehensive protocols for the capture of CTCs via filtration, density gradient centrifugation, microfluidic, immunomagnetic separation of CTCs, detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, flow ... Læs mere

1.868,14 kr.

This second editions explores new and updated approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Chapters detail comprehensive protocols for the capture of CTCs via filtration, density gradient centrifugation, microfluidic, immunomagnetic separation of CTCs, detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, flow
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Immunoproteomics

Methods and Protocols

Redigeret af Kelly M. Fulton, Susan M. Twine ...

Serier serie Springer Protocols
This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine. ... Læs mere

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This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine.
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Antibody Engineering

Methods and Protocols

Redigeret af Damien Nevoltris, Romain Ollier ...

Serier serie Springer Protocols
This fourth edition volume expands on the previous editions by providing readers with a comprehensive guide to the latest foundational and advanced techniques used by researchers in the field. This book covers the construction of diverse antibody libraries including naïve, synthetic, and immune types and candidate selection using phage, yeast, or mammalian display systems. This book also discusses ... Læs mere

1.681,32 kr.

This fourth edition volume expands on the previous editions by providing readers with a comprehensive guide to the latest foundational and advanced techniques used by researchers in the field. This book covers the construction of diverse antibody libraries including naïve, synthetic, and immune types and candidate selection using phage, yeast, or mammalian display systems. This book also discusses
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Regulatory T-Cells

Methods and Protocols

Redigeret af Masahiro Ono ...

Serier serie Springer Protocols
This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology ... Læs mere

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This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology
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Redigeret af Alejandro López-Soto, Alicia R. Folgueras ...

Serier serie Springer Protocols
This new edition explores the innovation and improvement of experimental techniques harnessed to dissect the complex relationship between cancer cells and the immune system of the host. Beginning with spectral and multicolor flow cytometric methods to characterize the immune contexture of different forms of metastases, the book continues with functional assays to study the activation status of ... Læs mere

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This new edition explores the innovation and improvement of experimental techniques harnessed to dissect the complex relationship between cancer cells and the immune system of the host. Beginning with spectral and multicolor flow cytometric methods to characterize the immune contexture of different forms of metastases, the book continues with functional assays to study the activation status of
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Peptide Antibodies

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Redigeret af Gunnar Houen ...

Serier serie Springer Protocols
This detailed new edition explores current methods for the production and use of peptide antibodies. The book delves into various aspects of peptide synthesis and analysis, peptide-carrier conjugation, epitope and paratope prediction and identification, as well as improved assays and other uses of peptide antibodies. Written for the highly successful Methods in Molecular Biology series, chapters ... Læs mere

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This detailed new edition explores current methods for the production and use of peptide antibodies. The book delves into various aspects of peptide synthesis and analysis, peptide-carrier conjugation, epitope and paratope prediction and identification, as well as improved assays and other uses of peptide antibodies. Written for the highly successful Methods in Molecular Biology series, chapters
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Antigen Processing and Presentation

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Redigeret af Efstratios Stratikos ...

Serier serie Springer Protocols
This volume discusses the latest advancements in the field of antigen processing and presentation, and the complex assays and techniques that have been devised to help pick apart molecular events along this pathway. The chapters in this book cover topics such as how to make and analyze MHC molecules and their cargo; analyzing the function chaperones that regulate antigen presentation; enzymes that ... Læs mere

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This volume discusses the latest advancements in the field of antigen processing and presentation, and the complex assays and techniques that have been devised to help pick apart molecular events along this pathway. The chapters in this book cover topics such as how to make and analyze MHC molecules and their cargo; analyzing the function chaperones that regulate antigen presentation; enzymes that
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This detailed volume expands upon the previous edition with key methods currently used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. New chapters explore the latest approaches for single cell B cell and T cell receptor sequencing, multiplexed immunophenotyping of lymphoma tissue samples, genetic manipulation and extended culture of ... Læs mere

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This detailed volume expands upon the previous edition with key methods currently used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. New chapters explore the latest approaches for single cell B cell and T cell receptor sequencing, multiplexed immunophenotyping of lymphoma tissue samples, genetic manipulation and extended culture of
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Ewing Sarcoma

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IMMUNO-model in Cancer

Tertiary Lymphoid Structures

B-Cell Receptor Signaling

Circulating Tumor Cells

Immunoproteomics

Antibody Engineering

Regulatory T-Cells

Cancer Immunosurveillance

Peptide Antibodies

Antigen Processing and Presentation

Lymphoma