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RNA Analysis

Methods and Protocols

Redigeret af Laurent Delpy ...

Serier serie Springer Protocols
This volume explores the latest technologies for RNA analysis, covering fundamental techniques and innovative approaches that expand our understanding of RNA biology. The chapters in this book are organized into four parts: reverse transcription and RNA capture methods; high-throughput sequencing methods; RNA maturation: splicing, polyadenylation, and epitranscriptomic modifications; and RNA ... Læs mere

1.774,73 kr.

This volume explores the latest technologies for RNA analysis, covering fundamental techniques and innovative approaches that expand our understanding of RNA biology. The chapters in this book are organized into four parts: reverse transcription and RNA capture methods; high-throughput sequencing methods; RNA maturation: splicing, polyadenylation, and epitranscriptomic modifications; and RNA
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Drosophila

Methods and Protocols

Redigeret af Christian Dahmann ...

Serier serie Springer Protocols
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

1.134,86 kr.

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and
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Chromoanagenesis

Methods and Protocols

Redigeret af Franck Pellestor ...

Serier serie Springer Protocols
This extensive volume explores the phenomena of chaotic genomic rearrangements, known as chromoanagenesis, their formation mechanisms, and the state-of-the-art techniques used to detect them. The book delves into the potential impact of chromoanagenesis on cancers and congenital diseases, as well as its broader implications for genomic evolution and human health through a variety of cutting-edge ... Læs mere

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This extensive volume explores the phenomena of chaotic genomic rearrangements, known as chromoanagenesis, their formation mechanisms, and the state-of-the-art techniques used to detect them. The book delves into the potential impact of chromoanagenesis on cancers and congenital diseases, as well as its broader implications for genomic evolution and human health through a variety of cutting-edge
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Cell Cycle Control

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Redigeret af Anna Castro, Benjamin Lacroix ...

Serier serie Springer Protocols
This detailed volume collects techniques to study the highly regulated cell cycle process. Beginning with chapters investigating these processes and assessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental conditions, different model systems and ... Læs mere

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This detailed volume collects techniques to study the highly regulated cell cycle process. Beginning with chapters investigating these processes and assessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental conditions, different model systems and
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Bacterial Secretion Systems

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Redigeret af Laure Journet, Eric Cascales ...

Serier serie Springer Protocols
This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or ... Læs mere

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This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or
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Redigeret af Véronique Arluison, Claudio Valverde ...

Serier serie Springer Protocols
This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary ... Læs mere

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Serier serie Springer Protocols
This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell ... Læs mere

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This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell
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This second edition volume discusses the latest techniques and protocols used in the field that were not covered in the previous edition. The chapters in this book are organized into five parts. Part One looks at transcriptomic analyses and Part Two covers DNA replication and protein/DNA interactions. Part Three discusses translation dynamics, protein complexes, and proteomics. Part Four looks at ... Læs mere

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This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into five sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the ... Læs mere

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RNA Analysis

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Drosophila

Chromoanagenesis

Cell Cycle Control

Bacterial Secretion Systems

Bacterial Regulatory RNA

Recombinant Protein Expression in Mammalian Cells

Yeast Functional Genomics

mRNA Decay

Genotype Phenotype Coupling

Methods for Plant Nucleus and Chromatin Studies

DNA-Protein Interactions